Fig. 1

IFN-γ induces galectin-9 expression at the mRNA and protein levels in HCC cells. a and b, HepG2 and Hep3B cells were treated with IFN-γ for 24 h at different concentrations, as indicated, and then galectin-9 and IRF1 protein expression levels (a) and mRNA expression (b) levels were analyzed by western blotting and qPCR, respectively. c and d, HepG2 and Hep3B cells were treated with 10 ng/ml IFN-γ for the indicated time period, and then galectin-9, IRF1 protein (c) and mRNA (d) expression levels were determined by western blotting and qPCR, respectively. e and f,HepG2 and Hep3B cells were treated with IFN-γ for different concentration and increasing time as indicated, EZH2 and H3K27me3 protein levels (e) and EZH2 mRNA levels (f) were detected by western blotting and qPCR, respectively. (g) HepG2 and Hep3B cells were treated with 10 ng/ml IFN-γ for 48 h, after which galectin-9 and EZH2 were subjected to immunofluorescence staining (magnification ×400; scale bar, 50 um). Cells treated with PBS were used as controls. The nuclei were stained with DAPI (blue), galectin-9 was stained with FITC (green), and EZH2 was stained with cy3 (red). (* P < 0.05, **P < 0.01, compared to PBS or 0 h group)