Fig. 4

ONC201 does not inhibit vascular growth in vivo. Relative fluorescent intensity from (a) Superhance 680 probe in HCT116 xenografts and (b) Angio-sense probe in MC38 tumors after 4 weeks. c Representative images of HCT116 GFP tumors. CD31+ cell count per 20× slide view for (d) Syngeneic models CT26 and MC38 and E) Human xenograft HT29 and MC38. e Representative CD31 image for HT28 xenografts. For all in vivo experiments, N = 5. RFI was measured using image j and calculated for (tumor-background)/tumor area. CD31 staining was performed on multiple slides per tumor for tumors of similar size. (e)CD31-HT29 xenografts