Fig. 3From: Autophagy promotes metastasis and glycolysis by upregulating MCT1 expression and Wnt/β-catenin signaling pathway activation in hepatocellular carcinoma cellsAutophagy promoted HCC cell glycolysis by upregulating MCT1. (a, b) Analysis of the consumption of glucose and production of lactate in HepG2 cells and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h. (c) The expression levels of glycolysis key enzymes in the starved HepG2 and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h were detected by real-time PCR. (d) Western blot showed the expression of MCT1 protein in the starved HepG2 and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h. Data are shown as the mean ± SEM of three independent experiments. (e, f) Real-time PCR and western blot were used to examine the expression of MCT1 in HepG2 and SMMC-7721 cells transfected with MCT1 siRNA. (g, h) Analysis of the consumption of glucose and production of lactate in HepG2 cells and SMMC-7721 cells cultured with EBSS for 6 h after transfected with MCT1 siRNA . Data are shown as the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01Back to article page