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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Autophagy promotes metastasis and glycolysis by upregulating MCT1 expression and Wnt/β-catenin signaling pathway activation in hepatocellular carcinoma cells

Fig. 3

Autophagy promoted HCC cell glycolysis by upregulating MCT1. (a, b) Analysis of the consumption of glucose and production of lactate in HepG2 cells and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h. (c) The expression levels of glycolysis key enzymes in the starved HepG2 and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h were detected by real-time PCR. (d) Western blot showed the expression of MCT1 protein in the starved HepG2 and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h. Data are shown as the mean ± SEM of three independent experiments. (e, f) Real-time PCR and western blot were used to examine the expression of MCT1 in HepG2 and SMMC-7721 cells transfected with MCT1 siRNA. (g, h) Analysis of the consumption of glucose and production of lactate in HepG2 cells and SMMC-7721 cells cultured with EBSS for 6 h after transfected with MCT1 siRNA . Data are shown as the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01

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