Fig. 2
From: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
INPP4B promotes cell proliferation in OCI-AML3 cells. (a) qRT-PCR and (b) western blotting analysis of INPP4B expression from the OCI-AML3 cells transduced with the control siRNA or siINPP4B. c CCK-8 assay analysis of cell proliferation activity in the siINPP4B transduced cells. d The OCI-AML3 cells stably infected with shRNA lentivirus targeting INPP4B were subjected to colony forming assays. e The INPP4B-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B protein levels and quantified using image software normalized against β-actin. f CCK-8 assay analysis of cell proliferation in INPP4B-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p < 0.05, ** p < 0.01, as comparison to mock group; # p < 0.05, as comparison to shINPP4B group