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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

Fig. 5

INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. (a) qRT-PCR analysis of INPP4B mRNA expression, (b) western blotting analysis of INPP4B, p-SGK3T320, SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. (c) qRT-PCR analysis of INPP4B mRNA expression, (d) western blotting analysis of INPP4B, p-SGK3T320, SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. (e) qRT-PCR analysis of INPP4B mRNA expression, (f) western blotting analysis of INPP4B, p-SGK3T320, SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. (g) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3T320 and SGK3, (h) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3T320, SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p < 0.05, *** p < 0.001

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