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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: RETRACTED ARTICLE: Exosomal microRNA-32-5p induces multidrug resistance in hepatocellular carcinoma via the PI3K/Akt pathway

Fig. 6

miR-32-5p promotes multidrug resistance through angiogenesis and EMT in vitro. (a-b) Western blots for the expression of N-Cad and E-Cad in Bel7402 and Bel/5-FU cell lines transfected with miR-32-5p mimics, inhibitor, siPTEN, or PTEN-expressing vector; co-transfected with miR-32-5p mimics and PTEN-expressing vector or miR-32-5p inhibitor and siPTEN; WM-treated Bel/5-FU cell lines transfected with miR-32-5p mimics, siPTEN, or respective NC. The expression of N-Cad and E-Cad are normalized to the level of the corresponding internal control β-actin. c Real-time PCR to analyze the expression of Twist and Snail mRNA in Bel7402 and Bel/5-FU cell lines transfected with miR-32-5p mimics, inhibitor, siPTEN or PTEN-expressing vector; co-transfected with miR-32-5p mimics and PTEN-expressing vector or miR-32-5p inhibitor and siPTEN; and WM-treated Bel/5-FU cell lines transfected miR-32-5p mimics or siPTEN. The levels of Twist and Snail mRNA are normalized to the level of the corresponding internal control GAPDH. n = three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA test. d ELISA for VEGF in the supernatant of Bel7402 and Bel/5-FU cell lines transfected with miR-32-5p mimics, inhibitor, siPTEN or PTEN-expressing vector, co-transfected with miR-32-5p mimics and PTEN-expressing vctor or miR-32-5p inhibitor and siPTEN, and WM-treated Bel/5-FU cell lines transfected with miR-32-5p mimics or siPTEN. n = three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test. e-g Transwell and wound healing assays in Bel7402 and Bel/5-FU cell lines transfected with miR-32-5p mimics, inhibitor, siPTEN or PTEN-expressing vector, co-transfected with miR-32-5p mimics and PTEN-expressing vector or miR-32-5p inhibitor and siPTEN, and WM-treated Bel/5-FU cell lines transfected with miR-32-5p mimics or siPTEN. n = three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test

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