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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The plant alkaloid tetrandrine inhibits metastasis via autophagy-dependent Wnt/β-catenin and metastatic tumor antigen 1 signaling in human liver cancer cells

Fig. 4

Autophagy-dependent Wnt/β-catenin pathway was involved in tetrandrine-inhibition of HCC cell migration. a Western blot analysis of p-GSK3β, total GSK3β, p-β-catenin, and total β-catenin protein levels after Huh7 and HCCLM9 cells were treated with DMSO or 2-μM tetrandrine (Tet) for 24 h. b Tetrandrine (Tet) decreases Wnt3a protein expression. c Overexpression of Wnt3a in Huh7 and HCCLM9 cells that were treated with DMSO or 2-μM tetrandrine (Tet) for 24 h. Wnt3a, p-β-catenin, total β-catenin and E-cadherin protein expressions were determined by western blot. d A transwell migration assay was performed on empty vector and Wnt3a-overexpression Huh7 and HCCLM9 cells that were treated with DMSO or 2-μM tetrandrine (Tet) for 24 h. The experiment was repeated three times, *p < 0.05. e Tetrandrine-inhibited Wnt/β-catenin activation was rescued by an autophagy inhibitor. Huh7 cells were pretreated with 3-MA (3 mM) for 1 h, followed by treatment with DMSO or 2-μM tetrandrine for 24 h; cell lysates were subjected to western blot analysis to detect the p-β-catenin and total β-catenin expression. f WT and ATG7-deficient Huh7 and HCCLM9 cells were treated with or without 2-μM tetrandrine for 24 h, followed by western blot analysis of p-β-catenin, total β-catenin, CyclinD1 and LC3 expression. GAPDH was loaded as an internal control

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