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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: The plant alkaloid tetrandrine inhibits metastasis via autophagy-dependent Wnt/β-catenin and metastatic tumor antigen 1 signaling in human liver cancer cells

Fig. 5

Tetrandrine inhibited HCC migration, in part through MTA1. a Expression analysis of MTA1 by quantitative real time-PCR and western blotting 24 h after treatment of Huh7 and HCCLM9 cells with or without 2-μM tetrandrine. Data are presented as the mean ± SD. of at least three independent experiments, *p < 0.05. b Huh7 and HCCLM9 cells were transfected with empty vector or MTA1 expression plasmid for 48 h. Cells were subsequently treated with DMSO or 2-μM tetrandrine (Tet) for 24 h, followed by western blot analysis of MTA1 and E-cadherin expression. GAPDH was loaded as an internal control. c A transwell migration assay demonstrated that MTA1 overexpression rescued tetrandrine-induced migration inhibition of Huh7 and HCCLM9 cells. *p < 0.05. d Huh7 and HCCLM9 cells were transfected with emptor vector or MTA1 overexpression plasmid for 48 h and then treated with DMSO or 2-μM tetrandrine for 24 h. Wnt signal related proteins, including Wnt3a, p-β-catenin, total β-catenin expression, and MTA1 levels, were detected by western blotting. e MTA1 expression was determined by western blotting after Wnt3a-overexpressed Huh7 and HCCLM9 cells were treated with or without 2-μM tetrandrine for 24 h. f Western blotting to detect MTA1 and LC3 expression after WT and ATG7-deficient Huh7 and MEF cells were treated with DMSO or 2-μM tetrandrine for 24 h

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