Fig. 4

miR-181b inhibits cell cycle distribution through CTDSPL and E2F1. a The miR-181b overexpression plasmid was stably transfected into MUM2b and OCM1a cells (MUM2b-over-miR-181b, OCM1a-over-miR-181b), and the plasmid also contained the EGFP tag. Obvious green fluorescence was observed in MUM2b-over-miR-181b and OCM1a-over-miR-181b cells, but not in the control groups (original magnification 100X). b The qRT-PCR results showed significantly higher expression of miR-181b of nearly 500-fold in MUM2b-over-miR-181b and OCM1a-over-miR-181b cells. c and (d) Cell cycle progression was significantly promoted in miR-181b-stably transfected MUM2b and OCM1a cells. The G0/G1 phase was significantly decreased by approximately 19% and 16%, while that the S-phases was significantly increased by about 10% and 18% in MUM2b-over-miR-181b and OCM1a-over-miR-181b cells, respectively, compared with the control group. e CTDSPL expression was significantly inhibited in miR-181b-stably transfected MUM2b and OCM1a cells. f E2F1 expression was significantly overexpressed in miR-181b-transfected MUM2b and OCM1a cells (*P < 0.05)