Fig. 4

VDAC3 acts as a tumor suppressor. Huh7 and HepG2 cells were transfected with pshR-VDAC3 or controls. HepG2.2.15 cells were transfected with pVDAC3 or controls. Twenty-four hours after transfection, cells were seeded into cell culture inserts. a MTT assays were performed to evaluate cell viability. b Colony formation assays were used to estimate cell proliferation. c Migration/invasion assays and f Western blot analyses were used to evaluate EMT. d VM and g molecular markers of VM were examined to assess tumor angiogenesis. e FACS assays were used to examine changes in cell cycle distribution. (*: P < 0.05)