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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: RETRACTED ARTICLE: miR-3928v is induced by HBx via NF-κB/EGR1 and contributes to hepatocellular carcinoma malignancy by down-regulating VDAC3

Fig. 6

HBx up-regulates miR-3928v expression via EGR1. a Schematic diagram of the location of miR-3928v in the human genome; the relative positions of the miR-3928v promoter constructs are shown (left). Luciferase reporter assays were used to examine miR-3928v promoter activity in Huh7 cells (right). b Promoter activity in HBV (+) and HBV (−) cells (left). Then, the promoter activity in Huh7 cells was examined after HBV or HBx overexpression (right). c The effect of EGR1 on promoter activity (left). The EGR1 binding site in the miR-3928v promoter was mutated, and the luciferase activity induced by EGR1 was examined (right). d ChIP analysis (left) and EMSAs (right) revealed that EGR1 directly binds to the miR-3928v promoter. e The miR-3928v expression level was measured by RT-qPCR after EGR1 overexpression or knockdown. f Blocking EGR1 inhibited HBx-induced miR-3928v promoter activity. g Representative IHC images showing the magnitude of EGR1 expression in tissues isolated from liver cancer patients. h Total and nuclear expression of EGR1 and P65 in Huh7 cells after transfection with HBx was analyzed by Western blot. i An immunofluorescence assay was used to detect the induction of EGR1 expression in the nucleus by HBx in Huh7 cells. j Mechanism by which HBx-induced miR-3928v expression contributes to HCC malignancy. (*: P < 0.05, **: P < 0.01)

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