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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: A synthetic cell-penetrating peptide derived from nuclear localization signal of EPS8 exerts anticancer activity against acute myeloid leukemia

Fig. 6

The inhibitory effects of CP-EPS8-NLS on EPS8 associated signaling. a U937, KG1α, HL-60 and TF1α cells were treated with increasing concentrations of CP-EPS8-NLS for 12 h, and analyzed by western blot to determine EPS8, Akt, p-Akt (473), p-STAT3, Erk and p-Erk levels. GAPDH expression was assessed to confirm equal protein loading. Densitometric analysis was performed using ImageJ software (NIH, Bethesda, MD; http://imagej.nih.gov/ij). b Western blot analyses of U937 and KG1α cells treated with 0, 35 or 105 μM CP-EPS8-NLS for 12 and 24 h were performed to examine mTOR, p-mTOR, p-Akt (308) and p-Akt (450) expression

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