Fig. 3

Upregulation TFAP2C promotes chemoresistance in CRC cells in vitro. a Annexin V-FITC/PI staining of the indicated cells treated with 5-FU (10 μM) for 36 h. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. b The JC-1 staining showed that upregulating TFAP2C increased, while silencing TFAP2C reduced the mitochondrial potential in the indicated CRC cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. c Western blotting analysis of BCL2 and BCL2L1 in the indicated cells. α-Tubulin was detected as a loading control. d and e Analysis of the activities of caspase-3 (d) and caspase-9 (e) were detected by the cleaved forms of these two proteins. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05