Fig. 7

TFAP2C transcriptionally upregulates ROCK1 and ROCK2 in CRC cells. a-c Real-time PCR analysis and Western blotting of ROCK1 and ROCK2 expression in the indicated CRC cells. α-Tubulin was detected as a loading control in the Western blot and GAPDH was used as endogenous controls in RT-PCR. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. d and e Analysis of ROCK1 and ROCK2 promoters physically associated with TFAP2C by using chromatin immunoprecipitation (ChIP) assay in the indicated SW480 cells. *P < 0.05. f and g Relative luciferase activity of the indicated promoter vectors in SW480 cells transfected with TFAP2C plasmids or siRNA. *P < 0.05