Fig. 5

DUXAP10 directly binds with PRC2, LSD1 and HuR in GC cells. a DUXAP10 expression levels in cell nucleus or cytoplasm fraction of GC cells were detected by qRT-PCR. U1 was used as a nucleus marker and GAPDH was used as a cytosol marker. b FISH was performed to determine the distribution of DUXAP10 in GC cells. c RNA–protein interaction prediction (RPISeq) analysis was used to predict the interaction between DUXAP10 and RNA binding proteins (http://pridb.gdcb.iastate.edu/RPISeq/), predictions with probabilities > 0.5 were considered positive. d DUXAP10 directly bound to PRC2, LSD1 and HuR in RIP assays using BGC823 and SGC7901 cell extracts. e Biotinylated DUXAP10 RNAs or its antisense sequence were incubated with BGC823 cell lysates, targeted with magnetic beads, and associated proteins were resolved electrophoretically. Western blot analysis of the specific association of SUZ12, LSD1, HuR and DUXAP10. AR mRNA interaction with HuR was used as positive control. *P < 0.05, **P < 0.01