Fig. 5

RHBDD1 influences the EMT and stemness signatures of HCT-116 cell line. a. Heatmap analysis of the pan-EMT signature in RHBDD1 knockdown and control HCT-116 cells. Mesenchymal genes are shown in the upper half, and the epithelial genes are shown in the bottom half. 1, 2, 3, different replicates. Ctrl, control; Si-pool, mixture of Si-RHBDD1–1# and Si-RHBDD1–2# in a ratio of 1:1. b. Differential expression genes in pan-EMT signature were analyzed. The Red line above the X-axis represents log2FoldChange > 1; the blue line beneath the X-axis represents log2FoldChange < − 1. Genes with log2FoldChange > 1 or log2FoldChange < − 1 were shown in black. c. Fisher's exact test of differential expression genes in mesenchymal genes and epithelial genes. Numbers (percentages) in the rectangles represent the numbers (percentages) of differential expression genes in indicated groups. *, P < 0.05. d. Heatmap analysis of the ISC signature in RHBDD1 knockdown and control HCT-116 cells. e. Differential expression genes in ISC signature were analyzed