Fig. 2

Up-regulation of SOX2OT variant 7 in OS tumor tissues and cell lines. Specific expression of SOX2OTs (variants 1, 4, 6, 7, and 8) in OS tumor tissues. a The expression of SOX2OTs in OS tumor tissue mixture was checked by qRT-PCR. The U-87 cell line was used as positive control. b qRT-PCR was performed to detect the SOX2OT V7 in 6 pairs of OS tissues (No.1–6), A means adjacent and T means timorous. Results shown are representative of three independent experiments and error bars indicate SE. ^* p < 0.05 vs. relative adjacent tissue

^** p < 0.01 vs. relative adjacent tissue. c qRT-PCR was performed to detect the mRNA level of SOX2OT V7 in 3 OS cell lines and primary osteoblast. Results shown are representative of three independent experiments and error bars indicate SE. ^* p < 0.05 vs. primary osteoblast group, ^** p < 0.01 vs. primary osteoblast group. d qRT-PCR was performed to detect the mRNA level of SOX2OT V7 in U2OS and SaoS2 cells with different treatment. Results shown are representative of three independent experiments and error bars indicate SE. ^* p < 0.05 vs. control group, ^** p < 0.01 vs. control group. ^# p < 0.05 vs. DOX single treated group