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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition

Fig. 4

EGCG reduced the expression of CSC markers of osteosarcoma stem cell. a OSCs obtained from osteosarcoma cell line U2OS by CD133+/CD44+ immunomagnetic beads double positive screening and the isolated CD133+/CD44+ OSCs were identified by flow cytometry analysis. U2OS parental cells were set as Control (Con). b The relative mRNA levels of CSC markers Nanog, Sox2 and OCT4 in different bead-sorted cells were checked by qRT-PCR. * p < 0.05 vs. parental cells, ** p < 0.01 vs. parental cells, # p < 0.05 vs. CD133+ cells. c qRT-PCR was performed to detect the SOX2OT V7 in different bead-sorted cells. * p < 0.05 vs. parental cells (Con). d Relative mRNA levels of CSC markers Nanog, Sox2, OCT4, c-Myc and ABCG2 in OSCs treated with or without EGCG were detected by qRT-PCR. * p < 0.05 vs. parental cells (Con), ** p < 0.01 vs. parental cells (Con), # p < 0.05 vs. OSC. Results shown are representative of three independent experiments and error bars indicate SD. e Expression of CSC markers Nanog, Sox2, OCT4, c-Myc and ABCG2 in OSCs treated with or without EGCG were detected by western blots

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