Fig. 5
SOX2OT V7 is one of the important targets of EGCG inhibiting OSCs. OSCs screened from U2OS and SaoS2 were infected with V7 overexpression lentivirus (Lv-V7) to generate gain-of-function OSC models of SOX2OT V7. a qRT-PCR was performed to detect the mRNA level of SOX2OT V7 in V7 overexpressed OSCs. ** p < 0.01 vs. control lentivirus infected OSCs (Lv-con). The sphere clone formation assay was performed to evaluate the self-renewal ability of OSCs. b 1 × 103 OSCs were inoculated in 96-well plate and number of spheres per well was calculated on day 14. * p < 0.05 vs. control lentivirus infected OSCs (Lv-con). c Representative pictures of a tumor spheres were taken on the day14, under 100 × magnification, the bar = 100 μm. d. MTT assay was performed to study the inhibitory effect of EGCG and DOX, alone or in combination on OSC growth. * p < 0.05 vs Lv-con OSCs which were treated under the same treatment condition