Fig. 1

The structure of dacomitinib and cytotoxicity of dacomitinib. The structure of dacomitinib (a). MTT cytotoxicity assay was assessed in ABCG2 and ABCB1-over-expressing cells and their parental sensitive cells: ABCB1-negative KB and ABCB1-overexpressing KBV200 cells (b); ABCG2-negative H460 and ABCG2-overexpressing H460/MX20 cells (c); ABCG2-negative S1 and ABCG2-overexpressing S1-MI-80 cells (d); ABCB1-negative HEK293/pcDNA3.1 and ABCG2-overexpressing Wild-type ABCG2-482-T7 and (e); ABCG2-negative HEK293/pcDNA3.1 and ABCG2-overexpressing mutant ABCG2-482-R2 cells (f). Cells were treated with varying concentrations of dacomitinib for 72 h. Results from three independent experiments were expressed as the mean ± SD