Fig. 2

Dacomitinib enhanced the anticancer effect of topotecan in the H460/MX20 cell xenograft model in nude mice. The changes in tumor volume over time after the H460/MX20 cell implantation (n =7) (a). Data shown are mean ± SD of tumor volumes for each group. The image of tumors size in four groups excised from the mice on the 25th day after implantation (b). Average percentage change in body weight after treatments (c). Mean tumor weight after excising from the mice on the 25th day after implantation (n = 7) (d). The four treatment groups were: (1) control (vehicle of dacomitinib, p.o., qod and saline i.p. qod); (2) dacomitinib (5 mg/kg, p.o., qod); (3) topotecan (2mg/kg, i.p., qod) and (4) topotecan (2mg/kg, i.p., qod) + dacomitinib (5 mg/kg, p.o., qod given 1 h before giving topotecan). Results were presented as the mean ± SD. * p< 0.05, ** p< 0.01