Fig. 3

Effect of dacomitinib on the intracellular accumulation of DOX, Rho 123 and Hoechst 33342 in MDR cells and their parental drug sensitive cells. The accumulation of Rho 123 (a), DOX (b) in S1 and S1-MI-80 cells and Hoechst 33342 (c) in H460/MX20 cells were measured by flow cytometric analysis as described in “Material and Methods” section. The results were presented as fold change in fluorescence intensity relative to control MDR cells. Columns, means of triplicate determinations; bars means SD. * p< 0.05, ** p< 0.01