Fig. 6

Effect of dacomitinib on AKT, ERK, and their phosphorylations in MDR and the parental drug sensitive cells. H460/MX20, S1-MI-80 and their parental drug sensitive cells were treated with different concentrations of dacomitinib, and 10 μM of lapatinib was used as positive control for the blockage of ERK phosphorylation for 48 h. The total and the phosphorylation protein level of AKT and ERK were detected by western blot (GAPDH as loading control). All these experiments were repeated at least three times