Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Integrin-β5, a miR-185-targeted gene, promotes hepatocellular carcinoma tumorigenesis by regulating β-catenin stability

Fig. 3

ITGB5 regulates β-catenin levels. a–b MHCC-97 L and Huh-7cell lysates were subjected to immunoprecipitation with control IgG, anti-ITGB5 (a) or anti-β-catenin (b) antibodies. The immunoprecipitates were then detected using the indicated antibodies. c–h Protein or mRNA levels of β-catenin in ITGB5-delepted or ITGB5-overexpressed stable cell lines were analysed by western blotting and q-RT-PCR. i–j Huh-7 and MHCC-97 L cells with or without ITGB5 knockdown were treated with MG132, and then, the protein levels of β-catenin were examined by western blotting. k–l ITGB5-delepted or ITGB5-overexpressed stable cells were treated with CHX for the indicated times and then, the protein levels of β-catenin were examined by western blotting. m–n ITGB5-delepted or ITGB5-overexpressed stable cell lines were transfected with HA-Ub and then treated with MG132 as indicated. Cell lysates were subjected to immunoprecipitation with anti-β-catenin antibody. The immunoprecipitates were then detected using the indicated antibodies

Back to article page