Fig. 1

Effect of 14, 15-EET on breast cancer cell adhesion and invasion. a 14, 15-DHET (a stable metabolite of 14, 15-EET) level in serum of BC patients was measured by ELISA. MCF-7 and MDA-MB-231 cells were untreated or treated with 14, 15-EET (100 nM) and/or 14, 15-EEZE (200 nM). b Intracellular levels of 14, 15-DHET in breast cancer tissues and paired adjacent noncancerous regions. c The adhesion ability of tumor cells was measured by adhesion assay. d The invasion ability of tumor cells was measured by Matrigel invasion assay. e Tumor cell arrest in lung and extravasation. Tumor cells were treated or untreated with 14, 15-EET (100 nM) and/or 14, 15-EEZE (200 nM) and labeled with CFSE, and then injected to mice via tail vein. Mice were sacrificed 5 h (for analysis of tumor cell arrest) and 24 h (for analysis of extravasation) after the i.v injection of CFSE-labeled cells. The CFSE-labeled cells in frozen sections were visualized by fluorescence microscopy. Fluorescent spots in the frozen sections of lung tissues were counted. *p < 0.05, **p < 0.01