Fig. 6

14, 15-EET induces tumor cells EMT and reduces cisplatin sensitivity of breast cancer cell in vivo. Nude mice were inoculated with MDA-MB-231 cells, tumors were developed in mice followed by treatment with 14, 15-EET and/or 14, 15-EEZE (i.v. injection, 30 μg/kg/2d). a Representative immunohistochemical staining of EMT marker. Nude mice were inoculated with MDA-MB-231 cells, tumors were developed in mice followed by treatment with 14, 15-EET and/or 14, 15-EEZE (i.v. injection, 30 μg/kg/2d). All mice were treated with cisplatin (i.p. injection, 3.0 mg/kg/d) or PBS. b The gross morphology of tumor samples. c The tumors volume was measured on the indicated days. d Tumors from mouse xenografts were removed and subjected to H&E staining and immunohistochemistry for Ki67. *p < 0.05