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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway

Fig. 4

Repression of GADD45α decreases the inhibitory effect of FL3 on cell cycle progression. a Flow cytometry assay was performed in T24 cells to measure the effect of FL3 on the cell cycle distribution in the presence or absence of siGADD45α-RNA. The percentage of cells in each phase was shown in the histograms; data represent the mean ± SD of three independent experiments, *P < 0.05 indicates a significant difference. b Total cell lysates from indicated T24 cells (up panel) were harvested and subjected to Western blot analysis with the indicated proteins (left panel). c Cell colony formation experiments were performed to measure the inhibitory effect of FL3 on cell proliferation of T24 cells in the presence or absence of GADD45α. Histograms represent the mean ± SD numbers of colonies of three independent experiments. **P < 0.01 indicates significance

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