Fig. 2
miR-193a accelerates cancer cell cycle and promotes cell proliferation and repopulation. a RT-qPCR for detecting miR-193a expression changes in the stimulated SW1990 and PANC-1 reporter cells co-cultured with their corresponding feeder cells (±X-ray). *p < 0.05, ***p < 0.001. n = 3. b Flow cytometry for assessing cell cycle in indicated cells. c EdU assay for detecting cell proliferation. Red, cells in proliferation stage. Blue, DAPI. Scale bar, 50 μm. d Repopulation of SW1990 reporter cells co-cultured with the corresponding feeder cells (±X-ray). n = 4. Left, representative bioluminescence images. Central, luciferase activity (photons/s). Right, representative fluorescent images. Scale bar, 1 mm. ***p < 0.001. e & f) miR-193a influenced pancreatic cancer cell proliferation in vivo. Nude mice were subcutaneously injected with SW1990-EgmiR-193a (E, 5 × 106) and PANC-1-hU6miR-193a-IN cells (F, 8 × 106). n = 5. Left & central, representative bioluminescence images in indicated time. Right, tumor growth curve of pancreatic cancer. ***p < 0.001, **p < 0.01