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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: microRNA-193a stimulates pancreatic cancer cell repopulation and metastasis through modulating TGF-β2/TGF-βRIII signalings

Fig. 3

miR-193a promotes cancer repopulation by regulating TGF-β2/TGF-βRIII/SMADs/E2F6/c-Myc signaling. a Schematic diagrams of the predicted miR-193a-binding sites in the 3’-UTR of TGF-β2, TGF-βRIII and E2F6. b The relative luciferase activity on 3’-UTRs, WT and MT counterparts were measured. **p < 0.01, ***p < 0.001. n = 3. c Western blot for detecting TGF-β2, TGF-βRIII and E2F6 proteins in SW1990 cells transfected with miR-193a angomir, and AsPC-1 cells transfected with miR-193a antagomir. d-g Western blot for detecting TGF-β2/TGF-βRIII/SMADs/E2F6/c-Myc signaling respectively in SW1990-EgmiR-193a and PANC-1-hU6miR-193a-IN cells (d), in SW1990-EgmiR-193a (±TGF-β2 cytokine, 10 ng/ml) and PANC-1-hU6miR-193a-IN cells (±TGF-β2 neutralization antibody, 2.5 μg/ml) (e), in stimulated SW1990 and PANC-1 reporter cells co-cultured with the corresponding feeder cells (±X-ray) (f), and in tumor tissues from burdened-mice (±X-ray) with SW1990-G2L cells (g)

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