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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: microRNA-193a stimulates pancreatic cancer cell repopulation and metastasis through modulating TGF-β2/TGF-βRIII signalings

Fig. 4

miR-193a destroys intercellular junctions and promotes cancer metastasis. a Immunofluorescence assay for observing E-cadherin and N-cadherin expression. Scale bar, 50 μm. b Transwell (Left, scale bar, 200 μm) and wound healing (Central & right, scale bar, 100 μm) assays for observing migration. c The penetration state of the stimulated SW1990 and PANC-1 reporter cells co-cultured with their corresponding feeder cells (±X-ray). Green cell (basal layer cell), HUVEC-G2L; Red cell (flow cell); SW1990-mcherry and PANC-1-mcherry reporter cells. d & e Liver metastasis of tumor-bearing mice with SW1990-EgmiR-193a cells d and PANC-1-hU6miR-193a-IN cells e. Upper, photograph of the dissected liver, n = 5. Scale bar, 1 cm. Lower left, liver metastasis rate. Lower right, numbers of liver nodules. *p < 0.05. f-i Western blot for testing TGF-β2/TGF-βRIII/ARHGEF15/ABL2 signaling in indicated cells

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