Fig. 3

MiR-10b directly targets CADM2 3′-UTR. a-b The mRNA and protein levels of CADM2 were determined by qRT-PCR and Western blotting after transfection with miR-10b mimic, NC mimic, miR-10b inhibitor, NC inhibitor in HEK293T cells. U6 or GAPDH were used as a loading control. c The predicted targeting sites of CADM2 3′-UTR binding to miR-10b were shown. MUT1: the first binding site was mutated; MUT2: the second binding site was mutated). d HEK-293T cells were co-transfected with wild-type 3′-UTR (WT) or mutant-type 3′-UTR (MUT1 and MUT2) reporters and NC mimic or miR-10b mimic. In the experiments shown in the panels, luciferase/Renilla activity was measured (P = 0.0459). The results are means ± SEM of three independent experiments. (*P < 0.05; **P < 0.01)