Fig. 4

MiR-10b promoted EMT process in HCC cell lines. a Expression of EMT markers in HepG2 cells transfected with miR-10b mimic, miR-10b inhibitor or its corresponding control were examined by immunofluorescence. (Green: E-cadherin, Red: Vimentin, Blue: DAPI) (b). E-cadherin and Vimentin expression were analyzed by Western blotting in HepG2 cells transfected with miR-10b mimic, miR-10b inhibitor or its corresponding control, GAPDH was used as a loading control. c A wound-healing assay was performed using HepG2 cells, and the photos were taken at 0 h and 48 h. d The Transwell assays with and without matrigel were performed in three HCC cell lines transfected miR-10b mimic, miR-10b inhibitor or its corresponding control. Representative photos for HepG2, Hep3B, Huh7 cells and quantitative analysis only for HepG2 are shown. Data represents mean ± SEM of three independent experiments. Error bars represent SD. (*P < 0.05; **P < 0.01; ***P < 0.001)