Skip to main content
Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Novel smac mimetic APG-1387 elicits ovarian cancer cell killing through TNF-alpha, Ripoptosome and autophagy mediated cell death pathway

Fig. 2

Effects of APG-1387 on apoptosis in ovarian cancer. a. APG-1387 inhibited the proliferation of SKOV3 cell line. They were treated with the indicated concentrations of APG-1387 for 24, 48, 72 h. Cell viability was determined by the CCK-8 assay. b Morphology of SKOV3 cells exposed to APG-1387(0, 10 nM) photographed under a fluorescence microscope (original magnification× 10). c APG-1387-induced apoptosis in SKOV3 cells was assessed by Hoechst33258 staining. Morphology of SKOV3 cells exposed to APG-1387 at different concentrations photographed under a fluorescence microscope (original magnification × 10). Condensated and fragmented nuclears were the mean ± SEM of 5 randomized areas. P < 0.01. d SKOV3 cells were treated with 10 nM APG-1387 for the indicated times. The cells were stained for phosphorylated H2AX and DAPI, then were analyzed by fluorescence microscopy (original magnification × 200). γ-H2AX positive spots were the mean ± SEM of 5 randomized areas. P < 0.01. e, f SKOV3 and OVCAR3 cells were exposed to various concentrations of APG-1387 (0, 10, 30 nM) for 24 h followed cell apoptosis analysis by flow cytometry. g Western blot analysis of caspase-3/PARP SKOV3 cells were treated withAPG-1387 (0, 3, 10, 30, 100, 300 nM) for 24 h. The data shown are representative of three different experiments. h SKOV3 cells were stimulated with APG-1387 for indicated periods of concentrations, caspase activation were tested by caspase activity assay

Back to article page