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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Novel smac mimetic APG-1387 elicits ovarian cancer cell killing through TNF-alpha, Ripoptosome and autophagy mediated cell death pathway

Fig. 4

APG-1387 is RIP1-dependent in ovarian cancer induced apoptosis. a Western blot analysis of the expression levels of caspases-8 and RIP1 at different concentrations of APG-1387 in cells. b Cells were treated with APG-1387 (10 nM) for the indicated times. The indicated proteins were detected by western blot after co-Immunoprecipitation with an antibody for RIP1, GADPH was detected as a control for specificity of co-Immunoprecipitation. c Western blot analysis of the effect of APG-1387 with or without addition of Z-VAD-FMK on RIP1 expression level in the SKOV3 cells. d Cells were transfected with caspases-8 siRNAs, cell viability was determined by the CCK-8 assay. e Cells were transfected with RIP1 siRNAs, cell viability was determined by the CCK-8 assay. f APG-1387 was coadministered with or without Necrostatin-1, cell viability was determined by the CCK-8 assay. g Cells were transfected with RIP1 siRNAs. Western blot analysis of the expression levels of caspases-3 and PARP. h The protein levels of cleaved-caspases-3/8 and PARP in cells by western blot after APG-1387 was coadministered with or without Necrostatin-1

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