Fig. 5

APG-1387 induces cell death partly through TNF-alpha signaling pathway. a ELISA analysis of the secretion of TNF-alpha after different time points of APG-1387 on SKOV3 cells streatment. b Cells were treated with different time points of APG-1387, and the secretion of TNF-alpha were analyzed by ELISA. c Cells were transfected with TNFR1 siRNAs, cell viability was determined by the CCK-8 assay. d Cells were transfected with TNF-alpha siRNAs, Cell viability was determined by the CCK-8 assay. e APG-1387 was coadministered with or without TNF-alpha neutralizing antibody, cell viability was determined by the CCK-8 assay. f Cells were treated with different concentrations of APG-1387 on cells after 24 h treatment and the expression levels of NF-κB1/p50 and NF-κB2/p52 were determined by western blot. g The protein levels of NF-κB1/p50 and NF-κB2/p52 in cells by western blot after APG-1387 was coadministered with or without Necrostatin-1