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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Efficient targeted tumor imaging and secreted endostatin gene delivery by anti-CD105 immunoliposomes

Fig. 2

Characterization of liposomes. a Rapid screening and quantitative analysis of 1-palmitoyl, 2-oleoyl phosphatidylcholine (POPC) in Lp by UHPLC-MS-MS. POPC had a maximum absorption peak at 205 nm, with m/z 761. A stock solution of POPC was prepared in chloroform, to a final concentration of 8 mg/mL. Working standards were prepared by serial dilution of stock solutions. Lp POPC was dried in a desiccator and extracted with 100% methanol. Chromatographic separations were carried out using a Shimadzu LCMS-8050 triple quadrupole mass spectrometer equipped with a Shimadzu Nexera X2 UHPLC system. In vitro cellular association and in vivo toxicity of Lp with or without pcDNA loading. b Confocal fluorescence images showing uptake of calcein-loaded Lp or ILp by primary tumor-derived microvascular endothelial cells (TECs; DAPI/nuclei, blue; calcein, green). Flow cytometry histograms of cellular fluorescence uptake. Negative control binding experiments were performed using isotype-matched controls or undecorated Lp. Total of 10,000 events based on the front scatter (FSS) and side scatter (SSC) gate were analyzed and displayed by colored histograms. Orange line, control; red line, Lp; blue line, ILp. Fluorescence micrographs of TECs transfected with pcDNA3.1-EGFP, Lp/pcDNA3.1-EGFP, and ILp/pcDNA3.1-EGFP

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