Fig. 3

The number of TIM3⁺ Tregs increased in the Tgfbr1/Pten 2cKO HNSCC mouse model. a Photographs of a wild-type mouse and a Tgfbr1/Pten 2cKO HNSCC mouse. b HE staining of squamous cell specific tumor tissue in the 2cKO HNSCC mouse model. c Flow cytometry analysis photographs of CD25⁺Foxp3⁺ cells gated from CD4⁺ T cells. d The percent of Tregs from the draining lymph node (LN) and spleen (SP) in WT (n = 6) and HNSCC (n = 6) mice. e Flow cytometry analysis photographs of CD206⁺F4/80 cells in the CD11b⁺ cell populations. f The ratio of CD11b⁺CD206⁺F4/80 macrophages from LN and spleen SP in WT and HNSCC mice. (mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, Mann-Whitney test)