Fig. 5

Impact of TIM3 blockade on immune checkpoints, M2 macrophages and IFN-γ production. a Percent of PD1, CTLA4, LAG3 and TIGIT expressed in the LN and SP of each group. b F4/80⁺CD206⁺ macrophages gated from the CD11⁺ cells in each group. c Percent of CD11b⁺F4/80⁺CD206⁺ macrophages in each group. d The flow cytometry photos of CD8⁺ T cells and IFN-γ⁺ production on CD8⁺ T cells (gated from CD3⁺ cells) in LN and SP of each group. e Percent of CD8⁺ T cells in each group. f Percent of IFN-γ⁺ cells in CD8⁺ T cells in each group (mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, Mann-Whitney test)