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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: TNFα induces Ca2+ influx to accelerate extrinsic apoptosis in hepatocellular carcinoma cells

Fig. 1

TNFα induces Ca2+ influx in HCC cells. a and b Confocal microscope analysis of [Ca2+]c using fluorescent probe Fura-2/AM in SNU739 and HLF cells with treatment as indicated. (Arrow: cells treated with TNFα or PBS). c and d Quantitative analysis of the maximal increased level of cytosolic Ca2+ after TNFα treatment (d) and (f) Confocal microscope analysis of [Ca2+]c in SNU739 and HLF cells with treatment as indicated before stimulation of 100 ng/mL TNFα. HBSS (Ca2+ free): cells cultured in Ca2+ free-HBSS before TNFα stimulation; HBSS (1.3 mM Ca2+): cells cultured in HBSS containing 1.3 mM Ca2+ before TNFα stimulation. Data were shown as mean ± SD. All experiments were performed at least three times. * P < 0.05; ** P < 0.01

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