Fig. 3

Decreased cytosolic Ca²⁺ level attenuates TNFα-induced apoptosis of HCC cells. a-d Cell apoptosis analysis by flow cytometry 24 h after treatment as indicated before stimulation of TNFα (100 ng/mL). BAPTA-AM: 10 μM; CAI: 10 μM; SKF96365: 100 μM; PV: expression vector encoding parvalbumin; siTRPM7: siRNA against TRPM7. e Tumor growth curves of subcutaneous xenograft tumor model developed from stable HCC cell lines (Right); dissected tumors from sacrificed mice (Left) were shown (n = 4 for each group). EV: xenografts developed from SNU-739 cells transfected with empty vector; PV, xenografts developed from SNU-739 cells stably forced expressing parvalbumin; TNFα: 40 μg/Kg. f TUNEL staining in tumor tissues of nude mice xenograft model with treatment as indicated. Blue: DAPI; Green: TUNLE positive nucleus. Quantification of cell death ratios were shown in the right. Scale Bar, 50 μm. Data were shown as mean ± SD. EV: empty vector; PV: parvalbumin. All experiments were performed at least three times. * P < 0.05; ** P < 0.01