Fig. 6From: TNFα induces Ca2+ influx to accelerate extrinsic apoptosis in hepatocellular carcinoma cellsCa2+ influx induced by TNFα activated calpain signaling to enhances extrinsic apoptosis. a Western Blot analysis for Calpain expression and analysis of Calpain activity after TNFα (100 ng/mL) stimulation for 4 h in HCC cells with treatment as indicated. b Western Blot analysis for expression of cIAP1, cIAP2, XIAP and cleaved caspase-3 after TNFα (100 ng/mL) treatment. c–h Analysis of Calpain activity and the expression of cIAP1, cIAP2, XIAP and cleaved caspase-3 after treatment with TNFα stimulation for 4 h in HCC cells with treatment as indicated. TNFα: 100 ng/mL; siATNFR1: siRNA target TNFR1; Calpeptin: 40 μM; PV: HCC cells transfected with expression vector encoding parvalbumin, Iono: 1 μM ionomyin. Data were shown as mean ± SD. All experiments were performed at least three times. * P < 0.05; ** P < 0.01Back to article page