Fig. 2

EEF1D Knockdown inhibits osteosarcoma cell growth in vitro. a-f Expression levels of EEF1D mRNA and protein in MNNG/HOS, U2OS and MG63 cells were measured after EEF1D siRNA (si-EEF1D) transfection by qRT-PCR and western blotting, respectively. g-i Cell Counting Kit-8 (CCK-8) assays were performed to measure cell proliferation after siRNA transfection. j-o Colony-formation assays were performed for EEF1D-silenced osteosarcoma and control cells. Data are representative of results from three independent experiments. * P < 0.05. For qRT-PCR, EEF1D expression was normalized to β-actin