Fig. 3

OGN increased dimerization, internalization, and degradation of EGFR. a HT29, SW620 cells with OGN over-expression were challenged with 100 ng/mL EGF for the indicated intervals. Cell lysates were subjected to Western blotting with indicated antibodies. b Immunofluorescent staining of cancer cells (B1: HT29, B2: SW620) with anti-EGFR antibody. Arrows show EGFR protein expression. c HT29, SW620 cells with OGN over-expression were treated with EGF (100 ng/mL) and co-cultured with or without MG132 (1 μM) for 24 h. d Cancer cells were treated with EGF (100 ng/mL) and then cultured with the cross-linker BS3 (3 mM) for 30 min at room temperature in order to demonstrate EGFR dimmers. e Recruitment of the endocytic adaptor Eps15 and epsin-1 is induced following OGN over-expression, when EGF stimulated for the indicated times