Fig. 2
From: Targeting KRAS in metastatic colorectal cancer: current strategies and emerging opportunities
EMICORON downregulates KRAS expression. a HCT116 colon cancer cells were treated with EMICORON at doses of 0.5 and 1 μM for 6, 12, 24 h. The DNA extracted was amplified by PCR. The histogram shows the relative optical density of KRAS. Histograms show the mean values ± SD. A representative picture of PCR products is shown. Densitometry was performed with ImageJ software version 1.40. b KRAS protein levels were detected by Western blotting. The total cell extracts of HCT116 cells treated with EMICORON 1 μM for 24 h, were prepared and immunoblotting was conducted by antibodies against KRAS (Santa Cruz) or Actin (Santa Cruz)