Fig. 3
CASZ1 inhibits HCC cell proliferation, migration and invasion in vitro a. The effects of CASZ1 on HCC cell proliferation was measured by MTT assay at different time points. b Representative imgages and quantification of cell clones in HCCLM3CASZ1, PLC/PRF/5shCASZ1 and their control groups, as determined by colony formation assay. c The effect of CASZ1 on HCC cell cycle progression was analyzed by flow cytometry. d Wound healing assay was performed to detect the migratory capacity of HCCLM3CASZ1, PLC/PRF/5shCASZ1 and their control cells. e Transwell invasion assay was performed to evaluate the invasive potential of CASZ1-interfered HCC cells. Each bar represents the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001