Fig. 5

CASZ1 inhibits HCC growth and metastasis via inactivating the MAPK/ERK signaling a. 10-Pathway Reporter Array was performed to detect the signaling changes in CASZ1-interfered HCC cells. **P < 0.01. b The expression of critical members and downstream effectors of MAPK/ERK pathway was examined by western blot in HCCLM3^CASZ1, PLC/PRF/5^shCASZ1 and their control cells. c The levels of p-ERK, MMP2, MMP9 and cyclinD1 were determined by western blot in HCCLM3^CASZ1, PLC/PRF/5^shCASZ1 and their control cells treated with U0126 (10 μM) or DMSO control. d-f. HCCLM3^CASZ1, PLC/PRF/5^shCASZ1 and their control cells were treated with U0126 (10 μM) or DMSO control and then subjected to MTT (d), wound healing (e) and transwell invasion (f) assays