Fig. 7

RAF1 is critical for CASZ1-mediated inhibition of MAPK/ERK signaling and HCC progression a. Western blot analysis of CASZ1, RAF1, p-ERK, MMP2, MMP9 and cyclinD1 expression in CASZ1-interfered HCC cells with RAF1 knockdown or ectopic expression. b-d. MTT (b), wound healing (c) and transwell invasion (d) assays were performed to test the influence of RAF1 on the proliferation, migration and invasion abilities of CASZ1-interfered HCC cells with RAF1 knockdown or ectopic expression. e Schematic depiction of the mechanism underlying CASZ1-mediated inhibition of HCC cell proliferation, migration and invasion based on our study