Fig. 6

Inhibition of Galectin-1 results in cell cycle arrest. ALL cells grown on (mitotically inactivated, irradiated) OP9 stromal cells were treated with DMSO or 10 μM PTX008, as indicated, for 72 h. a Representative flow cytometry images of BrdU and 7-AAD staining of LAX56 cells. Gates represent apoptotic/subG0 (Apop), G0/G1 phase (G0-G1), S phase (S), and G2/M phase (G2) of the cell cycle. b Overall cell cycle analysis of LAX56 and LAX57 cells. Graphs are combined data from 3 replicate experiments with 3 replicate samples. Error bars, standard error of mean (p<0.001, 95% CI, 2-way ANOVA). c, d. LAX56 treated for 72 h with 10 μM PTX008, 5 nM vincristine, or a combination of both and control, DMSO treated cells in the absence or presence of (mitotically inactivated, mitomycin-C-treated) OP9 stromal cells as indicated below the panels. Error bars, standard error of mean of triplicate samples. c Cell cycle analysis performed as in panel (a). (**, p<0.01, ***, p<0.001, ****, p < 0.0001 95% CI, 2-way ANOVA, control versus single or combination treatment). d Percentage of apoptotic cells measured by staining for Annexin V and 7-AAD (combination treatment compared to other samples **, p<0.01, ***, p<0.001, 95% CI, 1-way ANOVA)