Fig. 4

Overexpression of TUSC7 or inhibition of miR-224 inhibited chemotherapy resistance of ESCC cells. a EC9706 or KYSE30 cells transfected with pcDNA or pcDNA-TUSC7 were treated with cisplatin (0, 1, 2, 4, 8, 16 μM) for 48 h. Inhibition rate increased with the increase of concentration of cisplatin, and overexpression of TUSC7 increased the inhibition effect of cisplatin on ESCC cells, indicating overexpression of TUSC7 inhibited chemotherapy resistance of ESCC cells. b EC9706 or KYSE30 cells transfected with pcDNA or pcDNA-TUSC7 were treated with 5-Fu (0, 1, 4, 16, 32, 64 μM) for 48 h. Inhibition rate increased with the increase of concentration of 5-Fu, and overexpression of TUSC7 increased the inhibition effect of 5-Fu on ESCC cells. c TUSC7 level was detected in EC9706 and drug-resistant EC9706/DDP cells or KYSE30 and drug-resistant KYSE30/DDP cells. TUSC7 level was downregulated in drug-resistant ESCC cells. d EC9706 or KYSE30 cells transfected with pcDNA or pcDNA-TUSC7 were treated with 2 μM cisplatin for 48 h. Overexpression of TUSC7 promoted cisplatin induced apoptosis of ESCC cells. e EC9706 or KYSE30 cells transfected with NC or miR-224 inhibitor were treated with cisplatin (0, 1, 2, 4, 8, 16 μM) for 48 h. Inhibition rate increased with the increase of concentration of cisplatin, and miR-224 inhibitor increased the inhibition effect of cisplatin on ESCC cells, suggesting miR-224 inhibitor inhibited chemotherapy resistance of ESCC cells. f EC9706 or KYSE30 cells transfected with NC or miR-224 inhibitor were treated with 5-Fu (0, 1, 4, 16, 32, 64 μM) for 48 h. Inhibition rate increased with the increase of concentration of 5-Fu, and miR-224 inhibitor increased the inhibition effect of 5-Fu on ESCC cells. g miR-224 level was detected in EC9706 and drug-resistant EC9706/DDP cells or KYSE30 and drug-resistant KYSE30/DDP cells. miR-224 level was upregulated in drug-resistant ESCC cells. h EC9706 or KYSE30 cells transfected with NC or miR-224 inhibitor were treated with 2 μM cisplatin for 48 h. miR-224 inhibitor promoted cisplatin induced apoptosis of ESCC cells