Fig. 6From: LncRNA-TUSC7/miR-224 affected chemotherapy resistance of esophageal squamous cell carcinoma by competitively regulating DESC1TUSC7 inhibited cell proliferation and chemotherapy resistance via miR-224/DESC1. EC9706 or KYSE30 cells were divided into four groups: pcDNA, pcDNA-TUSC7, pcDNA-TUSC7 + NC, and pcDNA-TUSC7 + miR-224 mimic groups. a MTT assay showed that miR-224 mimic reversed the inhibition effect of pcDNA-TUSC7 on cell proliferation. b Colony formation assay showed that miR-224 mimic reversed the inhibition effect of pcDNA-TUSC7 on colony formation. c Flow cytometry showed that miR-224 mimic reversed the promotion effect of pcDNA-TUSC7 on cell apoptosis. d After the treatment of cisplatin (0, 1, 2, 4, 8, 16 μM) for 48 h, miR-224 mimic reversed the inhibition effect of pcDNA-TUSC7 on chemotherapy resistance. e After the treatment of 5-Fu (0, 1, 4, 16, 32, 64 μM) for 48 h, miR-224 mimic reversed the inhibition effect of pcDNA-TUSC7 on chemotherapy resistance. f pcDNA-TUSC7 increased protein level of DESC1 and decreased the expression of EGFR and p-AKT, while miR-224 mimic reversed these effectsBack to article page